HbA1c was assessed using a DCCT standardized reversed-phase cation exchange chromatography (HA 8160 analyzer, Menarini, Florence, Italy) Triglycerides, HDL-cholesterol and total cholesterol were determined from fasting plasma samples by enzymatic techniques (Boehringer-Mannheim, Mannheim, Germany).
Cholesterol and triglyceride concentrations in serum were assayed by enzymatic techniques.
We determined total cholesterol and triglycerides by enzymatic techniques, and calculated total serum lipid concentrations (Phillips et al. 1989).
Triglycerides, total cholesterol, and HDL cholesterol were determined from fasting plasma samples by enzymatic techniques (Boehringer-Mannheim).
Levels of fasting and postprandial glycemia, total cholesterol, HDL cholesterol and triglycerides were measured by enzymatic techniques.
Total serum cholesterol, high density lipoproteins (HDL), low density lipoproteins (LDL) and triglycerides were quantified by enzymatic techniques.
Levels of TAG, HDL-C, and glucose were measured by an enzymatic spectrophotometeric technique using Vitros 350 analyzer (Ortho-Clinical Diagnostics, Johnson & Jhonson, 50 100 Holmers Farm Way, High Wycombe, Buckighamshire, HP12 4DP, United Kingdom).
The serum levels of TC, TG, HDL-C, and LDL-C were determined by the standard enzymatic colorimetric technique, using Cholesterol CHOL-Pand(CHOL) and Triglyceride GPO-PAP (TG) Kits (Roche Diagnostics GmbH, Mannheim, Germany) and L-Type HDL-C and L-Type LDL-C Kits (Wako Life Sciences, Inc ., respectively.
FFA were measured by an enzymatic technique that used acylCoA oxidase (Wako Diagnostics, Wako Chemicals, USA , Inc (inter-assay CV of <1.5%).
The enzymatic restriction products were analyzed by electrophoresis on 10% polyacrylamide gel and detected by a nonradioisotopic technique using silver staining.
