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Serum creatinine and lipid profiles, including total cholesterol (TC), TG, HDL-c and low density lipoprotein cholesterol (LDL-c) were determined by enzymatic procedures using an autoanalyser (Hitachi 7600 020; Hitachi, Tokyo, Japan).
Plasma glucose, cholesterol and triglyceride levels were measured by enzymatic procedures using commercial kits (Elitech, Salon de Provence, France), by means of an automatic analyzer (Abbott VP, Rungis, France).
Glucose, ALT, and GGT were measured as part of a multichemistry profile (SMA20) by enzymatic procedures using the multichannel Olympus Au-5000 Analyzer (Olympus, Lake Success, NY).
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The plasma lipid profile was determined by measuring triglycerides and total cholesterol by standard enzymatic procedures using reagent kits (Hospitex Diagnostics, Florence, Italy).
Triglyceride (TG), total cholesterol (TC), high-density-lipoprotein (HDL) and low density-lipoprotein (LDL) were determined in plasma by standard enzymatic procedures using reagents kits (Hospitex Diagnostics, Florence, Italy).
Acetoacetate and β-hydroxybutyrate were assayed by means of standard enzymatic procedures using β-hydroxybutyrate dehydrogenase [ 21].
Fasting plasma glucose (glucose oxidase peroxidase method) and lipid profile (standard enzymatic procedures using Roche Diagnostic reagents, Mannheim, Germany) were estimated using the Hitachi 912 autoanalyzer.
Total cholesterol was determined by an enzymatic procedure using the automated cholesterol oxidase-phenol aminophenazone (CHOD-PAP) method.
22 Total cholesterol was determined by an enzymatic procedure using the CHOD PAP method at phases 3 and 7. Serum high-density lipoprotein (HDL) cholesterol concentrations were measured from the supernatant after the precipitation of non-HDL-cholesterol with dextran sulphate magnesium at phase 3 and with a direct homogeneous assay at phase 7, 23 using at both phases the CHOD PAP method.
Serum total cholesterol was determined by an automated enzymatic procedure using the Roche CHOD-PAP reagent agent, and HDL was measured with the Roche HDL cholesterol assay using PEG-modified enzymes and dextran sulfate.
Blood samples were used to determine serum total cholesterol by an automated enzymatic procedure using CHOD-PAP reagent (Roche Diagnostics).
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