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Hydrolysis conditions that maximize DOR release without significant degradation of both DEM and DOR were chosen and results were compared to those obtained by enzymatic method using β-glucuronidase.
The assay was done by enzymatic method using Randox Kit.
The decellularization of goat-lung tissue was done by enzymatic method using trypsin.
Plasma free fatty acid (FFA) levels were quantified by enzymatic method using a commercially available assay kit from Wako Chemicals GmbH, Germany.
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This process is mainly accomplished by enzymatic methods using cellulases.
Plasma glucose, urea and creatinine were measured by enzymatic methods using the Humalyzer Junior Semi-automated Chemistry Analyzer.
Total cholesterol, triglycerides (TG) and high-density lipoprotein cholesterol (HDL-c) were determined by enzymatic methods using commercial kits (Boehringer Mannheim, Germany).
Serum concentrations of triglycerides, total cholesterol (Infinity, Thermo Electron Corporation, Melbourne, Australia), free fatty acids (FFA) (Wako Chemicals, GmbH, Neuss, Germany) and glycerol (Sigma, St . Louis MO, USA) were measured by enzymatic methods using commercially available kits.
These analytes were measured by enzymatic methods using a chemistry analyzer.
Plasma total cholesterol and triglycerides were measured by enzymatic methods using reagents from Boehringer Ingelheim (Mannheim, Germany).
Serum concentrations of triglycerides and cholesterol were determined by enzymatic methods using reagents from Roche Diagnostics (Mannheim, Germany).
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