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The Ng081 pilE::PpilE gfpmut3 kan strain was constructed using a PCR fragment that contains fluorescent gene reporter with a selectable drug marker flanked by ends of the target gene.
We found a consistent pattern where SNPs were in fact sequencing errors when the region was covered only by ends of reads which is known to have poorer quality.
The amplified fragments were fused yielding the final fragment pilE::gfpmut3 kan, which was used for transformation.> The Ng095 pglF::PpilE gfpmut3 kan strain was constructed using a PCR fragment that contains fluorescent gene reporter with a selectable drug marker flanked by ends of the target gene.
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