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stephensi Indian strain by embryonic injection was performed at the University of California Irvine.
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For embryonic injection, mT/mG females were first plugged by Foxl2-CreER T2 males.
The ability of hatched larvae to swim upward was affected after embryonic injection with DCF and CBZ.
Generation and cloning of targeting constructs, embryonic injection and selection, clone verification, and generation of chimeric mice were conducted by InGenious Laboratories LLC.
Three injections were separated by 24 hours.
S.C. conducted embryonic injection assay for F0 mice generation.
One hour after the injection, embryonic brains were fixed with 4% paraformaldehyde.
Injection dose was verified following injection by standard plate count.
Thierry's embryonic business was saved by the sale of the 3,000 bottles of rosé.
Human embryonic material was provided by the Human Developmental Biology Resource (www.hdbr.org).hdbr.org
The construct, pUAST-myc-human Parkin was sent to the Transgenic Drosophila Fly Core (Charlestown, MA) for embryonic injections.
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