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The reaction was stopped by eliminating excess AHDAP, after crosslinking, by elution through a G25 MicroSpin column (Amersham) [10].
The reaction was stopped by eliminating the excess of AHDAP after crosslinking by elution through a G25 MicroSpin column (Amersham).
To this end, we prepared a detoxified Acai-1 by elution through a column of endotoxin-removing gel (denoted Acai-1ER).
The six sub-fractions were obtained by elution through a liquid chromatography column with a water stationary phase and an acetonitrile mobile phase, and separated into individual factions based on elution time.
Dust extracts were purified by elution through a glass column containing 4.0 g 6% deactivated alumina.
A25T was further purified by elution through a polymixin B-conjugated resin (Detoxi-gel endotoxin removing column, Thermo Scientific, Waltham, MA, USA) to remove possible endotoxin contaminants.
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The sepharose beads were washed 3 times with 800 µl ice-cold RIPA buffer and resuspended in 60 µl 2× sample buffer, followed by elution through boiling.
Human CD8+ T-cells were isolated similar to CD4+ T-cells by antibody depletion of all non-CD8+ cells followed by elution through iron-based MidimacsLS columns (MIltenyi Biotec).
Extracts were concentrated and cleaned by elution through two columns containing activated Florisil.
Extracts were concentrated and cleaned by elution through two columns containing Florisil.
Elution through a column under a magnetic field enriched the unlabeled CD4+ T cells.
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