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RNA integrity and quality were tested prior to use by electrophoretic separation using Bioanalyzer (Agilent Technologies).
Quality control was performed by OD measurements in a NanoDrop ND-1000 spectrophotometer (260/280 nm >1.8) and by electrophoretic separation using an Agilent 2100 Bioanalyzer (R.I.N. values >7.0).
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The extent of inhibition by the compounds is measured directly by quantifying the level of both unphosphorylated peptide substrate and phosphorylated product after electrophoretic separation using Caliper's LabChip assay technology.
In order to determine the type of proteinases contained in TES, samples were subjected to electrophoretic separation using representative substrate (gelatin) and pH 5 (7.6) conditions and proteolytic activity was developed by previous treatment of gels with proteinase inhibitors with selectivity for cysteine, serine, aspartyl, and metalloproteinases.
The purity and integrity of the RNA was verified by absorbance measurements at 260 nm with a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies Inc , USA and by electrophoretic separation with a Bioanalyzer using the RNA Nano kit (Agilent Technologies).
Polymerase chain reaction amplification using random-primed cDNA of 32 primary pancreatic cancer tissues was performed using oligonucleotide primers in the presence of [ αP]dCTP, followed by electrophoretic separation on 6% nondenaturing polyacrylamide gels both in the presence of 5% glycerol at room temperature and in its absence at 4°C.
The sizes estimated by electrophoretic separation were compared with the sizes determined by sequencing.
The PCR products were analyzed by electrophoretic separation in 2% agarose gel.
Analysis of the products of multiplex PCR is often carried out by electrophoretic separation or DNA melting curve analysis.
The integrity of total RNA was determined by electrophoretic separation on 1.2 % (w/v) denaturing agarose gels.
Chromatin fragmentation was checked by electrophoretic separation of DNA (decrosslinked and purified from either input chromatins or unbound fractions, as described above) on a 1.3% agarose gel.
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