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The clinical application of potentially effective biological therapies for common acute cardiac conditions, like myocardial infarction, has been limited by efficiency and specificity of delivery of therapeutic agents.
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Inspired by the high efficiency and specificity of repairing HBB −28 (A>G) mutation by base editing, we sought to correct HBB −28 (A>G) mutation in patient's cells.
In this study synthetic dual receptor targeted polyplexes were developed, mimicking biphasic cell entry characteristics of natural viruses to increase efficiency and specificity by a dual-receptor internalization mechanism.
The polyclonal anti-HCCR serum was tested for its efficiency and specificity by indirect ELISA and Western blot.
gambiae PEST sequence and at least two primer sets were used to assess their efficiency and specificity by running a standard curve over a five-fold serial dilution (Additional file 7).
CBMs are thought to increase the efficiency and specificity of the catalytic module by attachment to a specific sugar moiety [ 18– 20].
In conclusion, the efficiency and specificity of VPg uridylylation by picornavirus polymerases is greatly influenced by allosteric effects of ligand binding that are likely to be relevant during the viral replicative cycle.
The knockdown efficiency and specificity was verified by western blot analysis (Figure 4A).
The primers efficiency and specificity were tested by generating a melting curve (70°C and 95°C) and the PCR products were analyzed on agarose gel.
The biotinylation efficiency and specificity was examined by Western blot using HRP-streptavidin at 1 5000.
The primer efficiency and specificity were demonstrated by the overlapping amplification profiles and the melting curves of equal quantities of cDNA, respectively.
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