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Interestingly, this decrease in Survivin was not affected by ectopic induction of T-Antigen expression, which has been previously shown to up-regulate Survivin in JCV infected astrocytes and oligodendrocytes (23).
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Regulation of these genes by etsrp was confirmed by ectopic induction in etsrp overexpressing embryos and decreased expression in etsrp deficient embryos.
The immunohistochemical staining results suggest that most of this increase can be explained by de novo or ectopic induction of ZnT1 expression in regions other than the midgut constriction region [ 31].
This functional alteration of the nucleocytoplasmic barrier is likely induced by spore wall formation, because ectopic induction of sporulation signaling leads to premature dispersion of nucleoplasmic proteins.
Embryos expressing Etsrp-mcherry and flk:gfp were selected to test for ectopic induction for 23 of the 31 genes identified in Table 2. 21 of 23 genes were clearly induced ectopically by etsrp OE. Figure 2 shows the ectopic induction of the 14 genes identified below to be relevant to blood and/or vascular expression.
Interestingly, we did not find any premature or ectopic induction of NeuroM by MYC (Fig 2I L, U; Supplementary Fig S3ι κ).
To verify that the ectopic induction of Nkx2.2 expression by Pias1 was dependent on endogenous Gli transcriptional activity we took advantage of Gli3-Znf.
Ectopic induction of Hh signaling was achieved by utilizing R26-SmoM2 and CAGGS-CreER™ mice.
Ectopic induction of nonvascular/nonmyeloid target genes may also be the result of secondary effects of etsrp overexpression.
In contrast, ectopic induction of apoptosis can accelerate the movement.
These findings may indicate that the ectopic induction of several growth factors perturbs proper URS development.
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