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Restoring nuclear import by ectopic addition of a SV40 TAg NLS did not rescue the RNP biological activity, suggesting that PB2 interaction with α-importin is required for the biological activity of influenza polymerase, in addition to its role in protein transport.
This process can be simulated by ectopic addition of reactive oxygen species (ROS) in cultures without media withdrawal.
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Our in vitro data are supported by additional in vivo evidence for both ectopic addition of the H2A arginines as well as their removal in yeast and human cell line model systems, respectively.
Ectopic addition of MMP-1 (0.3 nM) caused a 2.8-fold increase in Rho-GTP activity, which was completely blocked by RWJ-56110 (Fig 5C).
The abolished cell-cycle arrest was restored upon ectopic addition of TIGAR protein using TIGAR cDNA (Supplementary Figure S4B).
Ectopic addition of 0.3 nM MMP-1 induced a twofold increase in endothelial cell permeability (Fig 4C).
This appears similar to the lacrimal glands where ectopic glands can be induced by addition of ectopic Fgf10 (Makarenkova et al., 2000).
We asked whether the overexpression of dMyc in Drosophila embryos causes an increase in apoptotic activity, and if this activity can be suppressed by the addition of ectopic miR-308.
Endogenous expression of miR-155 is expected to affect its target genes, and therefore few genes were expected to be differentially regulated by the addition of ectopic miR-155.
Furthermore, we show that involution can be induced by addition of ectopic ROS to fresh culture media.
In addition, excess growth signaling by ectopic activation of Tor signaling in wild-type larvae, was not sufficient to induce precocious autophagy.
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