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The luciferase activity produced by each sample was calculated relative to the activity produced by the prototype B95-8-LMP1 B95-8-LMP1 B95-8-LMP1
The score received by each sample was then averaged within the experimental group (trained, detrained and untrained), so that each group was assigned a total score between 0 and 7, with 0 corresponding to altered insertion site, unstained and not organized tendon, and 7 corresponding to a normal insertion site, stained and structurally well organized tendon.
The percentage inhibition of CL promoted by each sample was calculated using the following formula: 100 − [(AS/AC) × 100], where AS is the integrated area of each sample evaluated at each concentration, and AC is the integrated area of the control sample.
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On receipt by Nimblegen, each sample was subjected to additional quality control prior to processing for microarray analysis.
Parasite density measured by qPCR for each sample was calculated using International Standard (IS) as a calibrator32,33, which was used as factor to calculate the parasitaemia of each sample.
Service areas were generated by assuming that each sample was sent to its nearest laboratory (based on shortest road distance).
Excess adaptors were removed by AMPureXP beads and each sample was recovered in 10 μL of nuclease-free water.
Each of the resulting samples was analyzed by LC/MS: 10 µL of each sample was injected, and duplicate LC/MS analyses were performed for each sample.
The signal in each sample was quantified by measuring the absorbance of 450 nm by spectrophotometer.
Each sample was distinguished by different colors of lines.
Each sample was normalized by the cycle threshold geometric mean using reference genes rrsA and cysG59.
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