Sentence examples for by each primer set from inspiring English sources

Melt curves demonstrated that single products were amplified by each primer set.

Product identities generated by each primer set were subsequently confirmed by sequencing.

In other words, we simply calculated the percentage of taxa with more than 1% abundance in our benthic mixture that were detected by each primer set (Table 1).

Surprisingly, total pol III levels do not match with the relative RNA levels from the gene pools covered by each primer set (Additional file 3: Figure S3D).

Promoter annotation was performed by first identifying the sequences amplified by each primer set (Table 2) using the Ensembl Genome Browser and then inputting those sequences into Tfsitescan (http://www.ifti.org/).

Such variation is partly because of known differences in the range of HPV DNA types amplifiable by each primer set, and this was taken into consideration when estimating type-specific prevalence.

Reaction efficiency values were calculated by running each primer set on serial dilutions of a cDNA mixture comprising leaf and root material.

The production of a single PCR product was also verified by gel electrophoresis for each primer set.

We also tested the performance of each primer set by changing the number of PCR cycles.

The number of aptamers in the input was calculated from the cycle thresholds (CT) for each primer set by extrapolation with the standard curves made using 7500 System Software Applied Biosystemss, Foster City, CA, USA).

In a first step, all SNP assays used in this study were run on a dilution series of normal blood DNA to determine PCR efficiency (E) of each primer set by the formula E = 10−1/s, where s represents the absolute value of the slope in a plot of the threshold cycle (CT) against log of input amount.