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Five cir quartets, chosen at random, were analyzed per clade by each method described above (Additional file 6).
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The RT-PCR reactions were carried out at 95°C for 5 min followed by 40 cycles of 95°C for 15s and 60°C for 30s each by the method described previously by Dai et al., 2007 [ 24].
We tested whether m is explained only by the estimated error rate, given X by binomial testing for each cytosine residue, by following the method described by Lister et al. [ 47].
Glycogen and trehalose extraction was done simultaneously for each collected sample by using the method described by Parrou and François (1997) that is based on the alkaline digestion of yeast cells in 0.25 M Na2CO3 at 95 °C (Becker 1978).
In addition to measuring the cost of healthcare services provided by each hospital, the method described in this paper can be used in many other healthcare fields if the datasets are organized with cases in rows and time points in columns.
Total phosphorous was estimated by method described by Allens (1974).
The oil was analyzed for iodine, saponification and free fatty acid content by method described by the Association of Official Analytical Chemist [14].
Iron free BG11 was acquired by method described by [40].
ABTS radical scavenging activity was measured by method described by [ 37].
IC was measured by the method described by Hadcroft and Calverly [ 14] immediately before each forced inhalation.
Then, we calculate the signal subspace matrix F i and the noise subspace matrix W i from EVD of the correlation matrix (hat {boldsymbol {R}} omega _{i})) and estimate the DOA of incoming wideband signal sources by using WS-TOPS and each conventional method described in Section 4. The statistical performance was evaluated by performing 500 Monte Carlo runs for each algorithm.
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