Sentence examples for by each clone was from inspiring English sources

The recombinant VEGF isoform mRNA expressed by each clone was confirmed by real-time quantitative RT-PCR.

RNAi clones were sequence verified, and suppression by each clone was tested in five independent trials and shown to be statistically significant (P < 0.001, Fisher's exact test; Figure 1A).

In our study, we did not analyze clone-specific risk factors because we did not believe that we truly had an epidemic clone and because the number of patients affected by each clone was too small to allow a statistically significant comparison.

Following identification of cell type by immunofluorescence, each clone was scraped from the culture plate.

Types I through V were isolated from library C, VI from A, VII from B. To assess how well the genomic RFLP was represented by the cloned v-rDNA, each clone was digested with Bsp EI, which generated an internal fragment (Fig. 1D), and the resulting pattern was compared with that of the genomic Bsp EI RFLP in Southern analyses (Fig. 1F, Lane G).

To fingerprint the BAC libraries with WGP, the number of tags on each clone was determined by deconvolution by assigning the sequence reads to individual BACs.

Initial confirmation of each clone was by PCR with vector-specific primers.

The study was conducted to determine if mode of deployment (monoclonal versus clonal mixture) affected overall productivity and how or if each clone was affected by mode of deployment.

The fluorescence intensity of each clone was divided by its corresponding control, and then normalized to the expression of the VrActin gene.

The last lane is protein marker c The presence of chimeric proteins in the total cell proteins from each clone was confirmed by Western blot using anti-His antibody.

With respect to the qRT-PCR analysis results, the fluorescence intensity of each clone was divided by its corresponding control, after which the relative mRNA levels of CBF3 and COR47 after 0, 2 and 4 days of chilling stress were quantified using RT-qPCR for the seedlings in each of the ASA concentration treatments (0.1, 0.5, 1, 2 and 3 mM).