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Recombinants were easily identified by dsRed expression in the ocelli (expression in the eye is quenched by [white +]).
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We screened the F2 progeny to identify individuals in which the transgene marker (mini-white) segregated from the donor site chromosome and cosegregated with the receiver-site chromosome (distinguished by DsRed expression).
We obtained D. melanogaster transgenics for all five fosmids by selecting for the dsRed expression in the eye, which is easily identifiable in white- genetic background.
Interestingly, tissues with strong DsRed expression in AyFib-431a also expressed a high level of GFP in the hsp90-GFP strain.
Our findings suggested that the strong DsRed expression in AyFib-431a was mediated by the activity of a strong promoter/enhancer located at a single genomic locus.
Next, we examined DsRed expression in more detail.
The strong DsRed expression in the ovary was of interest as it suggested the possibility of maternal inheritance.
We grew seedlings of these lines and stained the leaves for GUS expression which showed a correlation with DsRed expression in seeds.
It could be argued that the DsRed expression in syncytia might influence the development of syncytia and lead to higher susceptibility or resistance.
To investigate this possibility, we crossed a female of AyFib-431a heterozygote with a male of the nontransgenic strain w-c and analyzed DsRed expression in the progeny.
The level of DsRed expression diminished in about half of the embryos by the late embryonic stage, i.e., around seven days after egg laying.
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