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Increased levels of heterologous protein accumulation and xylitol production were achieved by synthesizing the XR gene in the chloroplast codon bias and by driving expression of the codon-optimized XR gene using a 16S/atpA promoter/5′-UTR fusion.
We measured the effect of ectopic GluRδ2 in Golgi cells by driving expression of GFP and GluRδ2 with the L7 promoter (Fig. 7).
This was accomplished by driving expression of the UAS-TeTx transgene with TH-GAL4 that places the yeast transcriptional activator GAL4 under the control of the tyrosine hydroxylase (TH) locus [20], [31].
Synapses can be visualized in the 19 D type GABAergic motor neurons by driving expression of the synaptic vesicle marker synaptobrevin::GFP (SNB-1 GFP) SNB-1 GFPromoter of the C. elegans GAD (glutamic acid decarboxylase) gene, unc-25 [18], [19].
That these effects are due to the lesions in the acj6 gene was shown by the fact that restoring acj6 expression using a gene duplication or by driving expression of an UAS-acj6 transgene with acj6-GAL4 rescued the startle response.
Removal of FruC in either aSP4 or vAB3 by driving expression of fruC- shmiR with the TH and the pox9-1-6 driver, respectively, also resulted in a specific and strong loss of courtship in the dark.
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The muscle-attaching tendon cells are marked by sr.GAL4 driving expression of UAS.cherry::moesin (red).
The late larval lethality of Spc25c00064 homozygotes was prevented by a transgene driving expression of a Spc25-mRFP fusion protein under control of the Spc25 regulatory region (gSpc25- mRFP).
Expression is controlled by the lac promoter driving expression of T7 RNA polymerase which in turn acts on the T7 promoter to induce scFv expression.
By empirically comparing different strength promoters for driving expression of the 3 components and adjusting the number of lexA operators in the promoters of the LEU2 and lacZ reporter genes, we arrived at conditions that produced the desired result for the approach whereby low levels of constitutive FADD were complemented by expression of Fas (Figure 3C, 3D, S8).
p1339 contains a PGK-NEO cassette (phosphoglycerate kinase promoter driving expression of neomycin phosphotransferase) flanked by 2 loxP sites.
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