Sentence examples for by double staining using from inspiring English sources

The infarct size was evaluated by double staining using 1.5% Evans blue dye (Sigma) and 1% triphenyltetrazolium chloride (TTC, Sigma).

Characterization of cells expressing ERK, p38MAPKα and JNK was performed by double staining using cell-type specific antibodies.

Lineage analysis was performed by double staining using anti-mouseCD45.1 R-PE antibody with each of the following antibodies: CD4 (RM4-5.B) and CD8 53-6.7 53-6.7

Co-culture with hUCB-MSCs in the presence of A β42 demonstrated colocalization of microglia and NEP-expressing cells (red color) by double staining using antibodies against CD11b, a marker of microglia (green), and NEP.

Neuronal subpopulation expressing Dp71 was characterized by double-staining using presynaptic markers of mature excitatory (Type-1 vesicular glutamate transporter, VGLUT1) or inhibitory synapses GAD-65/67 GAD-65/67

Colocalization of ubiquitin- and FMRpolyG-positive inclusions was visualized and quantified by immunofluorescent double staining using antibodies against ubiquitin and FMRpolyG (8FM).

Alternatively, apoptosis was also quantified by flow cytometry after double staining using FITC-conjugated annexin V/propidium iodide (PI) apoptosis detection kit (Eppendorf, Milan, Italy), which allows discrimination between early apoptotic, late apoptotic and necrotic cell.

Quantitative evaluation of apoptosis was performed by flow cytometry after double staining using FITC-conjugated annexin V/Trypan blue (Eppendorf, Milan, Italy), which discriminates early apoptotic, late apoptotic and necrotic cells.

Apoptosis was evaluated by Annexin-V-FITC/PI double staining using the Apoptosis Detection Kit (Oncogene, San Diego, CA, USA), according to the manufacturer's instructions.

Apoptosis cells were quantified by annexin-V FITC annexin-V FITCiodide double standing using an Annexin-V/FITC kit (Bender MedSystems, Burlingame, CA, USA), according to the manufacturer's instructions.

Apoptotic cells were quantified by Annexin V-FITC and propidium iodide double staining using a staining kit from KeyGEN Biotech (Nanjing, China).