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These cells can be identified by double staining for CD31 (endothelial marker) and eGFP (reporter gene) as shown in (Figure 4C).

CX3CR1+ GC B cells isolated as above were then subjected to chemotaxis to CX3CL1 and migrated cells were characterized by double staining for CD23 and CD27.

In subsequent experiments, low density CD39− tonsil GC B cells were characterized immunophenotypically by double staining for CX3CR1 and a panel of GC B cell related markers.

The endothelial localization of BAMBI could be clearly shown by double staining for BAMBI and CD31 in mid-sized arteries and veins from mouse or human kidneys (Figure 4D and F).

Isolated mouse islets from mutant and WT animals were treated with 50 µM H2O2 for 2 h and β-cell apoptosis was analyzed by double staining for TUNEL and insulin.

In line with our observations in islets from Hq mutant mice, exposure of isolated human islets to siAIF resulted in a 8.8-fold increase in β-cell apoptosis (p<0,05), analyzed by double staining for TUNEL and insulin (Fig. 3B).

Postembryonically, stem cell organization was studied by double staining of root tips for expression of GUS driven by a QC-specific promoter trap, QC25[ 28], and starch staining of root cap cells.

The immunophenotype of transplanted cells was verified by double-staining for Nestin.

Altered pericyte/endothelial cell interaction due to NG2 ablation is accompanied by decreased pericyte maturation, as revealed by double-staining for desmin and the mature pericyte marker αSMA.

We quantified the number of Gems by double-staining for SMN and Gemin8, resulting in an average of 2.5 Gems per spinal cord motor neuron in control patients (Fig 3D).

COX-2 expression in tumour tissue from pancreatic cancer was examined by double staining immunohistochemistry for COX-2 and αSMA.