Sentence examples for by double selection on from inspiring English sources
After transformation, 33 independent transgenic T1 plants were obtained by double selection on Murashige and Skoog (MS) medium plates containing hygromycin and kanamycin.
The BY-2-TetR line was super-transformed with pTRAhDsRed-eTXvIL-10 and over a hundred independent callus clones were derived by double selection on medium containing kanamycin and hygromycin.
A MATα segregant from these dissections was mated with MAT a strains carrying relevant KanMX4-marked TS alleles of the "chemical suppression" candidates (ipl1 -1, ipl1 -2, spc105 -4, spc105 -15, tub4 -Y445D, erg26 -1, and ero1 -1) and zygotes were isolated by either micromanipulation or by double selection on YPD medium containing 200 μg/mL G418 and 100 μg/mL nourseothricin.
Finally, we select only a subset of these variables by using the "double selection" method of Belloni et al. (2014).
As expected, prestin/NubG-Alg5 did not grow on the quadruple dropout selective medium (SD-LTHA, Figure 2B), although both prestin and Alg5 were co-expressed by yeast as demonstrated on a double selection plate (SD-LT).
Similarly, the intersected genes between Pima developmentally regulated, TM1 developmentally regulated, and the top discriminating genes developed by double feature selection were clustered based on expression similarity.
Resistant seedlings were grown to maturity and progeny of these plants (T2) were grown on double selection again.
Progeny (T1) was grown on double selection: sulfadiazine for selection of the T-DNA insert in the DAYSLEEPER gene and PPT for selection of the construct with the shortened DAYSLEEPER gene.
As shown in Figure 3C and 3D, cdh23 bait interacts with NubI-Alg5 prey and grows on quadruple selection media (SD-LTHA) as shown in Figure 3D, but not with the negative control NubG-Alg5 prey, although both cdh23 and Alg5 were co-expressed by yeast as demonstrated in Figure 3C (SD-LT, double selection).
He discussed the hypothesis of double selection for NPC development that was introduced by Dolly Huang in 2004.
After 17 cycles of PCR amplification followed by double-size selection, the single-stranded paired-end library was then quantified by an Agilent 2100 BioAnalyzer on an RNA 6000 Pico LabChip and was measured to be 549 pg/μl.
