Sentence examples for by double immunofluorescence using from inspiring English sources
We then proceeded with the characterization of the intracellular localization of the two proteins in FRT cells by double immunofluorescence using antibodies against different intracellular organelle markers.
Treated and control tissues were characterized by double immunofluorescence using the mural cell marker α-SMA and CD31, while the ratio of desmin/CD31 was also determined by western blot.
Furthermore, dystonin-b antibody localized to the sarcolemma and intercalated discs as revealed by double immunofluorescence studies using vinculin antibody (Fig. 3).
By double immunofluorescence staining using human cellular immunophenotypic antibodies, 84 ± 16% of β-Gal-positive cells were also found to be positive for CD45.
The subcellular localization of the fusion proteins was examined by double immunofluorescence experiments using an antibody that recognizes the protein tags and Alexa 546-phalloidin to label actin filaments.
Initially, highly differentiated primary hypothalamic neuronal cultures were exposed to AβOs (500 nM) for 3 h and AβO binding to neurons was investigated by double immunofluorescence labeling using oligomer-sensitive antibody NU4 (Lambert et al, 2007) and microtubule-associated protein 2 (MAP-2).
EqS cell lines were analyzed by double labelling immunofluorescence using rabbit anti-PDGF βR and sheep anti-E5 primary antibodies (green fluorescence for E5 and red fluorescence for PDGF βR).
Carbonyl levels in the various CNS cell types were determined by double-label immunofluorescence using antibodies against NeuN, APC and GFAP to identify neurons, oligodendrocytes and astrocytes respectively.
Control and HIEC/shα8 cells were seeded on serum-coated glass coverslips and analysed by double-labelling immunofluorescence using an anti-vinculin antibody to detect focal contacts and phalloidin to stain actin microfilaments.
We further visualized the expression patterns of RASSF9 protein in mouse skin tissues by double immunofluorescence staining of frozen sections using RASSF9- and K1-specific antibodies.
The co-expression of α1D-AR and TRPV1 in tissue specimens from patients with adenocarcinoma or BHP, used as control, was evaluated by double immunofluorescence.
