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SSCP profiles of different SDV sites and an external field site in Germany were evaluated for their similarities and the contributing bacteria were characterized by DNA sequence analyses.
Successful incorporation of the mutations was confirmed by DNA sequence analyses, using forward and reverse primers to check the entire AQP1 sequence, and confirm the absence of random mutations.
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The mutations were confirmed by DNA sequencing analyses.
Accurate PCR reactions were confirmed by DNA sequencing analyses.
Changes in copy numbers were detected as well as single nucleotide polymorphisms (SNPs) by complementary DNA sequence analyses.
The plasmids were confirmed by restriction digests and/or DNA sequence analyses (University of Calgary Core Sequencing Facility).
This paper formally describes and names this species and provides further confirmation of its distinctive authenticity by presenting the results of additional DNA sequence analyses and cultural pairing studies.
DNA sequence analyses were conducted by a commercial sequencing service (Eurofins MWG Operon, Ebersberg, Germany).
After gene replacement, haploid strain 66132 carrying the hyqΔRI7 allele was isolated and verified by PCR and DNA sequencing analyses.
After gene replacement, haploid strains carrying deletion alleles were verified by PCR and DNA sequencing analyses [17].
Merodiploid strains 66205 and 66210 (Table 1) carrying both upstream hyqR::uidA+ hyqΔBI and downstream hyq+ operon were identified and verified by PCR and DNA sequencing analyses.
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