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Methoximation was performed by dissolving the samples in 50 µl of methoxyamine hydrochloride (20 mg/ml in pyridine) (Sigma, St . LouisMO, USA) to protect the carbonyls and incubating at 37 °C for 60 min.
Catalysts characterisation: Chemical analysis of Si, Al and Zr were performed in duplet by dissolving the samples in 1 % HF (48 % in H2O, 99.99 + % based on metal basis, Aldrich) and 1.25 % H2SO4 (99.999 %, Aldrich) solution and measuring them with inductively coupled plasma optical emission spectroscopy (ICP-OES) on a Perkin Elmer Optima 3000DV instrument.
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The Ag content of Ag/rGO nanocomposite was also determined by dissolving the sample in a concentrated HCl solution and analyzing the solution composition using a GBC SensAA Dual M/A Series Flame/Furnace atomic absorption spectrometer (AAS).
The Ag content of Ag/rGO nanocomposite was also determined by dissolving the sample in a concentrated HCl solution and analyzing the solution composition using a GBC Model SDS-270 atomic absorption spectrometer (AAS; GBC Scientific, Braeside, Australia).
The metals (Nb, Zr, Ti, Al) and Si composition of the catalysts were analyzed by elemental analysis (ICP-AES M/s Thermo Scientific iCAP6500 DU) by dissolving the sample in aqua regia along with a few drops of hydrofluoric acid in a microwave oven for 2 h and further diluted with de-ionized water to analyze the metal (Nb, Zr, Ti, Al) content.
Immediately prior to GC-MS analysis, derivatization was performed in two steps: Firstly, methoximation was carried out by dissolving the sample in 50 µL of 20 mg/mL solution of methoxyamine hydrochloride in pyridine to protect the carbonyls.
NMR samples were prepared by dissolving the sample in ∼0.3 0.6 mL of methanol- d4 (D, 99.95%).
Stock solution was prepared by dissolving the sample in sterile water at a concentration of 50 mg/ml and stored at 4°C, as described earlier [ 19].
Total lipid aliquots (2 mg) were subjected to acid catalyzed transesterification by dissolving the sample in 1 mL toluene, employed to ensure that the neutral lipids got properly dissolved, plus 2 mL of a mixture of MeOH/1% H2SO4, and incubated in a capped glass test tube at 50°C for 16 h [ 50].
The solutions were prepared by dissolving the ITR samples (from all irradiation doses 0 400 kGy) in methanol to a concentration of 0.02% w/ v.
Concentrations ranging from 5 to 800��μg/mL for antibacterial activity tests, and 50 to 2000 μg/mL for antifungal activity tests were employed by dissolving the test samples in DMSO.
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