Sentence examples for by direct tetramer analysis from inspiring English sources

Seven out of 10 patients had CEA-specific SKILs detected by direct tetramer analysis, whereas none of the patients had antigen-specific T cells in the peripheral blood.

Since a direct comparison of HIV-specific CD4+ T cells by tetramer analysis and cytokine production is yet to be reported due to the difficulties in producing stable peptide-MHC class II tetramers, it is unknown what proportion of HIV-specific CD4+ T cells are defective in IL-2 or IFN-γ production in acute and chronic infection [18].

In general, approximately 30% of the infused CTL clones identified in vivo by tetramer analysis were capable of secreting IFN-γ by ELISPOT analysis (adjusted for CD8+/PBMC ratio).

The research team has shown that survivin-specific T cells can be measured ex vivo in the peripheral blood of patients with head and neck squamous cell carcinoma by tetramer analysis and from the tumor-draining lymph node of a patient with locally advanced breast cancer by ELIspot analysis.

In four out of seven patients, we found CEA-specific T cells by means of tetramer analysis.

We further characterized the SQLLNAKYL-specific CD8+ T cells by combining MHC tetramer analysis with intracellular staining for IFN-γ and GrB.

A total of 6/10 patients (table 1) with resolved infection demonstrated CD8 T cell responses to the NS3 1080–88 TVYHGAGTK epitope by tetramer analysis (figure 4A,B).

10.7554/eLife.05949.019 Author response image 1. Exclusion of false-positive cells by two-color and dump tetramer analysis modeled with TCR negative T cell hybridoma.

In the current study, macaques vaccinated for >2 years with BCG do not exhibit P65-specific CD4 T cells that can be confidently detected by intracellular IFN-γ staining or by direct DR*W201/P65 tetramer staining.

This is borne out by direct analysis.

For tetramer analysis, cells were stained with Db Smcy PE-conjugated tetramer as described 45.