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Cytofluorimetric analysis was done by direct staining for CD4 fluorescein isothiocyanate (FITC-conjugated YTS 191.1.2 mAb; Immunotools, GmbH, Germany) or CD8 (PE-conjugated YTS 169.4 mAb; Immunotools) and was always >95%.
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Next, the cultures were stained for F-actin by direct staining with Alexa 488-phalloidin (Molecular Probes, OR, USA) for 1 h.
The increased number of anti-inflammatory Treg cells infiltrating the spinal cord in EPO-treated EAE animals was confirmed by direct immunohistochemical staining for Treg cells (mAb against Foxp3+) on paraffin-embedded spinal cord sections.
Further, impression cytology from other sites like conjunctiva and skin vesicles can be collected by this method, especially for direct staining techniques.
Amplification products were visualized by gel electrophoresis or direct staining of DNA.
All phospho-specific markers and PCNA stains were evaluated by direct reciprocal dual-staining for the test antigen with in combination with antibodies to ErbB2 and α-SMA to verify that all ErbB-2 negative responses were identical to α-SMA positive responses and vice versa.
CD11b on the cell surface was detected by direct immunofluorescence staining.
The specific lymphocyte antigens were determined using direct staining with monoclonal antibodies and analysed by flow-cytometry.
Detection of 'sulphur' granules by gram or histologic staining is the principal method of direct detection of Actinomyces.
In the current study, macaques vaccinated for >2 years with BCG do not exhibit P65-specific CD4 T cells that can be confidently detected by intracellular IFN-γ staining or by direct DR*W201/P65 tetramer staining.
Rabies was diagnosed by direct fluorescent antibody staining.
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