Sentence examples for by direct sequencing using from inspiring English sources

The amplification products were validated by direct sequencing using an automated DNA sequencer (373 DNA sequencer, Applied Biosystems, Foster city, CA, USA).

In order to validate the credibility of the identified eSNP, nucleotide sequences of PCR products containing the eSNP regions were determined by direct sequencing using a DNA sequencer ABI-37300 xl, Applied Biosystems, Foster City, CA, USA).

The identities of PCR products were confirmed by direct sequencing using an automatic DNA sequencer (PE Applied Biosystems).

Mutation status of KRAS gene (exon 2 and 3), PIK3CA gene (exon 9 and exon 20), BRAF gene (exon 15), was investigated by PCR amplification followed by direct sequencing using ABI 3730 automated sequencer (life Technologies).

The gaps in the assembly after high-throughput sequencing were filled in by direct sequencing using the ABI 3730 DNA sequencer by Sangon Biotech (Shanghai) Co., LTD., using adjacent PCR primers.

Furthermore, the amplified products were isolated by electrophoresis on a 1% agarose gel and then purified using the QIAamp purification kit (Qiagen Inc ., and the nucleotide sequence was determined by direct sequencing using an ABI 310 automatic sequencer according to the manufacturer's instructions (Applied Biosystem, Foster City, CA, USA).

TP53 mutation analysis [ 17] was carried out by individual PCR amplification of each of exons 4 to 10 followed by direct sequenceing using the Applied Biosystems BigDye Terminator v3.1 cycle sequencing Kit (Life Technologies) on an ABI PRISM 3100-Avant Genetic Analyser (Life Technologies) [ 18].

The complete genome sequence was determined by a direct sequencing using the products of RT-PCR, 5'RACE, and 3'RACE as sequencing templates [ 10].

The presence of the TTN-c.43628insAT mutation was verified in control and patient PSFs and iPSCs by PCR and direct sequencing using 50 ng of genomic DNA (Genomic DNA Purification Kit; Gentra Systems).

Genomic DNA was extracted from frozen or paraffin-embedded tumours and mutational analysis of PIK3CA was performed by PCR and direct sequencing using primers for exons 9 and 20, as previously described (Gallardo et al, 2012).

We detected the male-specific substitutions to reveal the genetically diverged regions between neo-sex chromosomes by PCR and direct sequencing using genomic DNA from two-to-six males and four females.