Sentence examples for by direct sequencing technology from inspiring English sources

Few case reports identified pHR-HPV73 and 82 DNA in L-SCC by direct sequencing technology (Pannone et al, 2010; Si-Mohamed et al, 2012).

This highlights the importance of pursuing alternative, direct sequencing technologies.

We identified and characterized microRNAs and endogenous siRNAs in S. japonicum both in the schistosomula (14 days post infection) and in the adult worms by direct sequencing using Solexa sequencing technology, as this technique achieves a more complete coverage of small RNA transcripts than a traditional cDNA cloning approach[ 7- 9].

The identity of the PCR products was confirmed by direct sequencing (ABI prism 310 technology).

Mutation screening was performed by direct sequencing using Big Dye terminator technology (ABI 3130), and sequences were analyzed using Bioedit packages.

Mutation status of KRAS gene (exon 2 and 3), PIK3CA gene (exon 9 and exon 20), BRAF gene (exon 15), was investigated by PCR amplification followed by direct sequencing using ABI 3730 automated sequencer (life Technologies).

Improvements in the throughput of NGS technologies made it possible to conduct SNP typing of an entire mapping population by direct sequencing, combining the process of polymorphism discovery and genotyping in a single experiment.

Polymorphisms were analyzed by direct sequencing.

rs671 frequency in each group was assessed by direct sequencing.

Deleted sequences were confirmed by direct sequencing of gel-purified PCR products (Supplementary Information tab 5).

SNP genotyping was performed by direct sequencing of purified PCR products and DNA sequencing, or by TaqMan analysis57.