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PCR-amplified P510S sequence specificity was checked by direct sequencing of the amplified product by the automated DNA sequencer A.L.F.
Deleted sequences were confirmed by direct sequencing of gel-purified PCR products (Supplementary Information tab 5).
MPL mutations, in particular W515L, W515K, W515A, S505N and G509C, were tested by direct sequencing of exon 10.
SNP genotyping was performed by direct sequencing of purified PCR products and DNA sequencing, or by TaqMan analysis57.
PCR results were confirmed by direct sequencing of the second round amplicons.
HCV genotype is subsequently determined by direct sequencing of the DNA fragment generated from the QPCR assay.
Genes encoding ESBLs were detected by polymerase chain reaction (PCR) amplification followed by direct sequencing of PCR products.
Further examination of the HCV genotype by direct sequencing of the PCR product showed a classical lb genotype sequence.
The p53 mutation status was analyzed by direct sequencing of the PCR product in highly conserved exons 5 8.
The correct integration of the late ASFV p72 promoter regulated GFP expression cassette was confirmed by direct sequencing of PCR amplicons spanning the mutagenized locus.
Finally, this was confirmed by direct sequencing of PCR product from line ST12 and 12 recipients (Fig. 2a).
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