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Samples of "no-mutation detected" were further analyzed by direct sequencing for identification of the major haplogroup to which they belong.
A first panel of rabbits used for mapping was genotyped by direct sequencing for the deletion using primers F9/R9 (Table 3).
We first analysed the 114 routine samples by direct sequencing for K-Ras mutation detection.
FCGR2A genotyping was performed by direct sequencing for >80% of samples.
Loci Le006861 and Le016258 were investigated by direct sequencing for the 31 varieties and accessions.
For the majority of amplicons, dHPLC is used as a mutation screening test, followed by direct sequencing for characterisation of mutations indicated by dHPLC.
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The ND4L T10652C variant was confirmed by direct sequencing of mtDNA for the highest heteroplasmic individual (81% C heteroplasmy by microarray calculation) within the D1 haplogroup who was an MDD subject and had the C allele (Fig. 2).
No further relevant mutations were found by direct sequencing of the genes for APOA5, APOC2, LMF1 and GPIHBP1.
Genotyping accuracy in the samples was confirmed by direct sequencing of PCR products for 5% of randomly chosen samples.
Genotyping accuracy in the samples was confirmed by direct sequencing of PCR products for 5% randomly chosen samples.
Conventional reverse transcription PCR was performed on RNA extracted from stool samples to detect rotavirus (4 ) and NoV genogroups I (GI) and II (GII) (5 ), followed by direct sequencing of the amplicons for genotyping.
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