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Detected mutations were validated by direct sequence.
Genomic sequences of mutant and wild-type viruses were confirmed by direct sequencing.
RT-QPCR primers were designed from the respective sequences and confirmed by direct sequencing as described.
Most data were obtained by direct sequencing.
Sequences of all mutants have been confirmed by direct sequencing.
DQA1 and DQB1 alleles have been determined by direct sequencing.
Each of these fragments was further analyzed by direct sequencing.
All insert fragments were confirmed by direct sequencing.
GBV-C genotypes were determined by direct sequencing.
‡Obtained by direct sequencing.
Verify mutation by direct sequencing.
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