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Visualization is achieved by fluorescently labeling either the DNA or the protein of interest, followed by direct imaging using high-sensitivity fluorescence microscopy.
9c Assembly was confirmed by agarose gel electrophoresis and by direct imaging using negative-stain transmission electron microscopy (TEM; Figure S3).
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(A ) Bacteriophage λ dsDNA (48.5 kbp) was biotinylated, denatured, coated with SSBAF488, and then (B ) attached to a streptavidin-coated glass coverslip of a microfluidic chamber where it was extended by buffer flow for direct imaging using total internal reflection fluorescence (TIRF) microscopy.
The coexistence of different micellar morphologies was visualized by direct imaging by cryoTEM.
Lgl was visualized by direct imaging of the fluorescent protein.
Iliac veins were visualised by direct imaging and Doppler flow.
Hence, by direct imaging, HS-AFM could provide irrefutable evidence for the Huxley's hypothesis.
BAT's relative activation following ICV BMP8B was further confirmed by direct measurement using thermal imaging and correlated extremely well with changes in core temperature in a given animal.
Because electron lenses allow subatomic resolution, direct imaging can be used.
Transduction efficiency was analyzed 24 h after infection through GFP detection by direct microscopy imaging and Flow Cytometry using a FACScalibur® (BD Biosciences, Franklin Lakes, NJ, USA).
Unpurified and purified samples were analyzed using agarose gel electrophoresis and direct imaging by transmission electron microscopy (TEM; Figure 1 b,c).
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