Ferritin was obtained by immunological agglutination, serum iron was obtained using a FerroZine method without deproteinization, and unsaturated iron binding capacity was obtained by direct determination using FerroZine.
To achieve this, liquid - liquid extraction (LLE) was employed for purification and concentration followed by direct determination using LC-MS/MS.
Furthermore, kinetics studies revealed that the cell-permeable INr peptides enhance the integration process, as was indeed confirmed by direct determination of viral DNA integration by real-time PCR.
FC migration was measured by two methods: staining with an anti-FC rabbit antibody [17] superimposed to the phase contrast image to visualise flagella or by direct determination of the contact point between old and new flagella as seen by phase contrast or by MAb25.
Kidney function was estimated as single kidney GFR (skGFR) by direct determination of clearance of chromium ethylenediaminetetraacetic acid (Cr-EDTA).
Furthermore, the first finding of urinary DON, ZEN, their conjugates, and OTA in this region and an advanced understanding of toxicokinetic patterns by direct determination of conjugation and hydroxylation products of DON and ZEA was achieved [ 38].
Indeed, direct determinations of Bacteroidetes content (qPCR) were generally higher than indicated by the clone libraries.
The prepared nanomaterial showed remarkable electrocatalytic ability as a result of the rational conjunction of graphene and gold nanoparticles, which was demonstrated by direct electrochemical determination of three aminophenol isomers on a modified glassy carbon electrode.
Branch thickness was calculated by direct thickness determination, as described previously (Hildebrand and Rüegsegger, 1997).
Pollutants were hourly collected by direct gravimetric determination method for PM10, by chemiluminescence for NO2, by ultraviolet fluorescence spectroscopy for SO2, and by infrared-ray absorption for CO.
