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Ferritin was obtained by immunological agglutination, serum iron was obtained using a FerroZine method without deproteinization, and unsaturated iron binding capacity was obtained by direct determination using FerroZine.
To achieve this, liquid - liquid extraction (LLE) was employed for purification and concentration followed by direct determination using LC-MS/MS.
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Kidney function was estimated as single kidney GFR (skGFR) by direct determination of clearance of chromium ethylenediaminetetraacetic acid (Cr-EDTA).
Structure determination of polymorph 1-B HT was then successfully accomplished by direct space methods using the TOPAS-academic program.
Miscibility of cyclic olefin copolymer/polyolefin elastomer (COC/POE) blends over full composition range was investigated through determination of viscoelastic characteristics both at melt and solid state as well as by direct morphological analysis using experimental and theoretical approaches.
0.2 ml was used for direct determination of androstendione and 0,5 ml for direct determination of corticosterone.
Certified reference materials (CRMs) of different origin were used to validate the direct determination of total mercury by solid sampling Zeeman atomic absorption spectrometry (SS-ZAAS) and a specially designed furnace.
However, most electrodes using nanostructures for the direct determination of biomolecules such as H2O2 still have several problems unsolved.
In the present study, conventional and multivariate methods were used to optimize conditions for direct determination of aluminum in soft drinks by electrothermal atomic absorption spectrometry.
Enzyme activities were determined using highly specific and sensitive assays that measured I− release as well as direct determination of iodothyronine metabolite production by HPLC [29].
These findings indicate that a direct determination of the concentration via cell counting should be used when exact values are needed, since the relation between optical density and concentration is limited by the pigmentation of the cells.
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