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Topoisomerase I levels were quantitated in crude extracts from colorectal, prostate, and kidney tumors and their matched normal counterparts by Western blotting and by direct determination of catalytic activity, and mRNA levels were determined by Northern blotting.
Ferritin was obtained by immunological agglutination, serum iron was obtained using a FerroZine method without deproteinization, and unsaturated iron binding capacity was obtained by direct determination using FerroZine.
To achieve this, liquid - liquid extraction (LLE) was employed for purification and concentration followed by direct determination using LC-MS/MS.
Furthermore, kinetics studies revealed that the cell-permeable INr peptides enhance the integration process, as was indeed confirmed by direct determination of viral DNA integration by real-time PCR.
FC migration was measured by two methods: staining with an anti-FC rabbit antibody [17] superimposed to the phase contrast image to visualise flagella or by direct determination of the contact point between old and new flagella as seen by phase contrast or by MAb25.
Kidney function was estimated as single kidney GFR (skGFR) by direct determination of clearance of chromium ethylenediaminetetraacetic acid (Cr-EDTA).
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Application of the method to tap water and two different river water samples resulted in values well confirmed by direct determinations with ET-AAS.
Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples.
In other words, it is the very independence of aesthetic response from direct determination by concepts, including moral concepts, that makes the experience of beauty an experience of freedom that can in turn symbolize moral freedom.
In contrast with cellular electrophysiology, where individual cells can be patched or penetrated by electrodes for the direct determination of membrane potential, indirect techniques must be employed for the much smaller mitochondria.
For evaluating the yields, a new method by Fourier transform infrared spectrometry (FTIR) has been developed for the direct determination of TiO2 by absorbance measurements in KBr pellets.
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CEO of Professional Science Editing for Scientists @ prosciediting.com