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Cell number was then determined by direct counting using a Neubauer chamber.
Allele frequencies were calculated by direct counting using the PyPop software [ 37].
The cell concentrations were ascertained by direct counting using Trypan blue.
The cell growth rate was determined by direct counting using randomly collected microscopic pictures.
Cell proliferation assays were performed by direct counting using a bacterial counting chamber (Hausser Scientific Partnership, PA, USA) and differential interference contrast microscopy.
The cell density (cells/mL) was determined by direct counting using a Neubauer chamber and a light microscope equipped with a 40× objective.
Similar(52)
Spore production was determined by direct counts using a Petroff-Hauser chamber.
From this sample 10 µl was taken for direct counts using an epifluorescent microscope.
(C) Cell number was estimated by direct cell counting, using the Trypan blue method.
Cell densities were determined by direct cell counting using acridine orange epifluorescence microscopy (Hobbie et al., 1977).
We used a standard curve of known concentrations of cells (determined by direct cell counting using trypan blue in a haemocytometer) to validate the use of this assay as a representative measure of cell viability in this cell line.
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