We show here, using photoaffinity and fluorescent derivatives, that this inhibitory effect is mediated by direct binding of cyclopamine to the heptahelical bundle of Smoothened (Smo).
All bacteria used prevented VSV binding to the cell monolayers by direct binding of VSV to their surface.
Two different mechanisms were considered: the disruption of Aβ aggregation by direct binding of P7C3 to Aβ and alterations in amyloid precursor protein processing through the inhibition of β-secretase.
When applied to yeast ChIP-chip data, our method reveals that only 48% of the data sets can be readily explained by direct binding of the profiled TF, while 16% can be explained by indirect DNA binding.
A Ulex europeus agglutinin I (UEAI -reactive glycoprotein(s) with molecUEAI -reactiveglycoprotein0,000 was detected by direct binding of 125I-labeled UEAI to lysates of rectal or sigmoid colon cancer tissues separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.
Mechanistically, this regulation is conferred by direct binding of cGMP to the CNBD, which is allosterically coupled to the HCN channel activation gate.
It is not known whether this effect was mediated by direct binding of cAMP-CRP near the promoter regions of the LEE genes.
Inhibitory functions of regulatory T cells are mediated by direct binding of CTLA-4 and by cytokine production [28], [50], [51].
This activity is mediated by direct binding of ZNF9 to the internal ribosome entry site sequence (IRES) within the 5'UTR of ODC mRNA.
However, PKDs can also be activated by direct binding of diacylglycerol (DAG) to the C1a domain within their regulatory domain [17].
Their method revealed that only 48% of targets could be explained by direct binding of the profiled transcription factors, while 16% could be explained by indirect binding.
