Sentence examples for by direct assay using from inspiring English sources
High-density lipoprotein cholesterol (HDL-C) was quantified by direct assay using enzymes modified by polyethylene glycol and dextran sulphate.
The caspase enzyme activity in the cell lysates (treated and control) was measured by direct assay using synthetic fluorogenic substrate (Ac-DEVD-AFC, substrate for caspase 3 (R&D Systems) and Ac-LEHD-AFC, substrate for caspase 8 and caspase 9.
High-density lipoprotein cholesterol (HDL-C) was quantified by a direct assay using polyethylene glycol modified enzymes and dextran sulfate.
Plasma NPY was measured by direct assay without extraction using a competitive radioimmunoassay (RIA) from ALPCO Diagnostics (Salem, NH).
However when we aligned these proteins with an experimentally known P. falciparum protein kinase (PF11_0220, protein kinase activity, Molecular Function GO 0004672, evidence code: Inferred by Direct Assay (IDA), source: PlasmoDB release 9.0) using PfFSmat60 matrix [ 9, 10], the alignments were significantly lengthier (Additional File 8) suggesting these proteins were probably protein kinases.
Cell culture supernatants were also tested by a direct immunoperoxidase assay using anti-influenza A and anti-influenza B nucleoprotein antibodies [ 20].
Viral load in the supernatants of tissue homogenates was assessed by direct plaque assays using LLC-MK2 cells.
The effectiveness of this method was proved by direct microbiologic assays using BHI and Sabouraud Agar mediums to bacteria and fungi growth, respectively.
In some experiments, apoptosis was determined by direct TUNEL labelling assay using In Situ Cell Death Detection Kit, Fluorescein (Roche Applied Science, Indianapolis, IN, USA) following the manufacturer's instructions.
In parallel, in order to evaluate the presence of dead cells, cell growth was also measured by direct cell counting assay, using a Neubauer hemocytometer chamber and according to trypan blue dye exclusion test.
