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Phage samples were prepared in TN buffer by diluting each sample to a uniform concentration of 2.0 × 10 PFU/mL; 150 μL of each prepared sample was then added to a sterile 96 well plate (Starstedt) and were analyzed using a SpectraMax M5 spectrophotometer at an excitation of 485 nm and an emission of 555 nm.
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Samples were withdrawn at several different times for quantitation of heat-resistant spores (survivors of incubation at 60°C for 20 minutes) and total CFUs by serially diluting each sample (in water for spore CFUs or SBB for total CFUs) and plating each in duplicate on BHIS agar supplemented with 0.1% taurocholate.
After diluting each sample by adding 70 μl of water, 2 μl were used in 50 μl semi-quantitative PCR reactions with 26 cycles, unless stated otherwise.
Athletes have been known to manipulate tests by using substances that mask the presence of performance-enhancing drugs, by using the urine of others or by diluting the sample with substances such as water.
Calibrators are prepared by diluting the sample and adding analyte with negligible uncertainty to fit in a concentration range where signals are homoscedastic.
Negative matrix effects due to the complexity and lipophility of the studied matrix were reduced by diluting the sample with distilled water.
The Au/[Bmim][PF6] nanofluids with concentrations of 2.55 × 10-4 and 5.1 × 10-4% were also fabricated by diluting the sample 4 before the TC measurements.
SM-LATE-PCR was carried out by diluting the sample to slightly less than the theoretical value of one molecule per well.
For cultures containing CaCO3, OD was determined by diluting the sample 6-fold with 4 N HCl to solubilize CaCO3.
After dissolving the dried sample with 100 μL of methanol, a stock solution was prepared by diluting the sample 10 times.
Samples were prepared for MALDI-TOF analysis by diluting the sample solution in the matrix-saturated solution to reach a 10 pmol/μL concentration.
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