The sensitivity of this MSP, obtained by diluting a completely methylated sample into a nonmethylated sample, was 10% (data not shown).
Four different sample concentrations were prepared by diluting a high concentration of HBaqs with toluene as the solvent.
We normalized matrix interference effects by diluting all samples and standards into the same solution, which contained 10% (v/v) translation solution.
Amplification efficiencies, analytical sensitivity, and accuracy of gene assays were measured by serially diluting an RNA sample and analyzing features of the linear regression between RNA expression measured by the crossing point (Cp) versus the log2 of the RNA input per PCR assay well.
The RNA was thereafter reversed transcribed into cDNA by diluting the sample in a mixture containing 10× RT buffer, 25× dNTP, 10× RT random primers and 10× multiscribe RTase (Applied Biosystems, Foster city, California, USA; code: 4368813).
Phage samples were prepared in TN buffer by diluting each sample to a uniform concentration of 2.0 × 10 PFU/mL; 150 μL of each prepared sample was then added to a sterile 96 well plate (Starstedt) and were analyzed using a SpectraMax M5 spectrophotometer at an excitation of 485 nm and an emission of 555 nm.
After dissolving the dried sample with 100 μL of methanol, a stock solution was prepared by diluting the sample 10 times.
Lipoplexes were prepared in phosphate buffered solution (PBS), for subsequent determination of size and zeta potential by dynamic light scattering (DLS) and laser Doppler anemometry, respectively, using a Zetasizer Nanoseries ZS (Malvern Instruments) by diluting the sample 50x with dd-water.
A secondary confirmation was obtained by diluting analytical samples by a factor of two and ensuring that the resulting concentrations were similarly halved.
Samples were prepared for MALDI-TOF analysis by diluting the sample solution in the matrix-saturated solution to reach a 10 pmol/μL concentration.
