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The heart rate was determined by digital motion analysis and power spectral analysis through extraction of frequency characteristics of the cardiac rhythm.
Analysis of cardiac performance by digital motion analysis requires immobilization of animal samples because any movement could result in complication of subsequent data analysis.
In summary, we have developed a rapid, simple and non-invasive method to determine the heart rate and heartbeat regularity from the peripheral circulation of zebrafish embryo by digital motion analysis and power spectral analysis.
From these video sequences, the heart rate and appendage beating rate was determined by digital motion analysis as described elsewhere [ 73]. cSNARF-1 fluorescence spectra were obtained from the hemolymph space around the heart region.
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A custom made program was developed in C# language (Visual Studio.NET, Microsoft) to implement the method of digital motion analysis developed by [ 20].
Clusters of markers on the thorax, scapula, clavicle and humerus enable the digital motion analysis system to provide comprehensive kinematic analysis of various patterns of movement.
In the present study, we described a simple, non-invasive methodology by video-recording under stereomicroscope and analyzing the video records with our cuctom made software, which integrated digital motion analysis [ 20] and power spectral analysis, to determine the heart rate.
In the original procedures of digital motion analysis, embryos were placed in horizontal position and thus movement of blood cells was along the axis parallel to the lines of CCD chip [ 20].
Additionally, kinematic data were collected by a motion analysis system (OptotrakCertus, Northern Digital, Waterloo, ON, Canada).
Kinematics data were collected by a motion analysis system (OptotrakCertus, Northern Digital Inc., Waterloo, ON, Canada).
Kinematic data were collected by a motion analysis system (Optotrak Certus, Northern Digital, Waterloo, Canada).
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