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31 Synovial tissue biopsies were collected from each patient, stored and processed for immunohistochemistry, and stained sections analysed by digital imaging analysis as previously described.
Tumor material was successfully incorporated in a tissue microarray in 76% of patients (93 of 121) and morphometric quantification by digital imaging analysis was possible in 94% of those cases.
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Nonstaining bands representing the levels of the latent forms of MMP-2 and MMP-9 were quantified by densitometer measurement using a digital imaging analysis system.
Using digital imaging analysis a vascular area score was recorded.
Analysis of microcirculatory parameters using OPS imaging as well as fluorescence microscopy were performed off-line from the videotapes by digital image analysis system (Cap-Image, Ingeneurbuero Zeintl, Heidelberg, Germany).
Images were evaluated by digital image analysis using the MetaMorph image analysis package (Universal Imaging, Downingtown, PA).
Sections were analysed by digital image analysis.
Stained sections were evaluated by digital image analysis.
The sections were evaluated by digital image analysis.
The stained slides were evaluated by digital image analysis.
Immunohistochemical analysis was performed and stained sections were analysed by digital image analysis.
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