Elemental content of bulk soils and redox masses were measured by digesting the samples in aqua regia and HF solution (EPA Method 3052 [44]).
DNA was extracted from 40- μm unstained sections of FFPE samples by digesting the samples in proteinase K (Fluka, Buchs, Switzerland) for 48 h, boiling in 5% chelex (Sigma-Aldrich, Dorset, UK) and phase extracting through chloroform-and-ethanol precipitation (Coombs et al, 1999).
Total Kjeldahl nitrogen was estimated by digesting the compost sample in concentrated sulphuric acid and by distillation and trapping the ammonia in 0.1 N sulphuric acid titrated against standard 0.1 N sodium hydroxide solution (AOAC 1995).
Total N content was analyzed by Kjeldahl's method after digesting the sample with a digestion mixture (sodium sulfate and CuSO4) and concentrated H2SO4 (Jackson 1967).
A RRL was prepared by digesting the pooled DNA sample with Sau3A and isolating the fragments in the size range of 2-3 kbp.
Check the integrity of the amplified repeat by digesting the remaining 10 μl sample from Step 62 with an endonuclease that cleaves the array into its original repeat unit (see Figure 1e).
Total potassium (TK) and Total calcium (TCa) were determined by flame photometer after digesting the samples.
This adduct (18 in Scheme 4) most likely represents the same label, but in this case, the 3-oxopropanoate moiety, a β-keto acid, has lost CO2. 5 The site of the modification was first localized to a peptide fragment of Cg10062 by digesting the modified and unmodified Cg10062 samples with protease V-8 and analyzing the resulting peptide mixtures by MALDI-MS.
We were able to overcome this by re-digesting the samples following PCR, then running the samples on an agarose gel.
We generated reduced representation libraries (RRLs) from 17 grapevine DNA samples (10 cultivated V. vinifera varieties, 6 wild Vitis species and the reference genome (inbred Pinot Noir) – see Table S1 for details on samples) by digesting each sample with the restriction enzyme HpaII, which has proved useful in the generation of RRLs by others [26], [27].
