Sentence examples for by digesting the plasmid from inspiring English sources

The DNA fragment encoding the promoter sequence of S. cerevisiae PGK1, secretion signal sequence of SAG1, and 3′ half of the α-agglutinin gene, including the terminator sequence, was prepared by digesting the plasmid PGK406 AG using the restriction enzymes Xho I/Not I (Yamakawa et al. 2012).

The insert was released by digesting the plasmid with KpnI and EcoRI, purified, ligated into the pRSET expression vector (Invitrogen), and used to transform DH5α cells.

Then, the DNA fragment containing His-tagged recC-ORF was released from pETC by digesting the plasmid with XbaI and SacI, and ligated into pGL10 to generate pGC.

The correct plasmid was verified by digesting the plasmid with the restriction enzymes EcoRI/ XbaI, AvrII, MfeI/ SnaBI, and PflMI.

First, the HindIII restriction site of pMSCVpuro was deleted by digesting the plasmid with HindIII, blunting the 3' recessed ends and re-ligating.

Linear template DNA was generated by digesting the plasmid with restriction enzyme NotI and RNA was produced by in-vitro transcription using T3 RNA polymerase.

To create the HDHBc-tdimer2 retroviral expression vector the open reading frame consisting of the carboxy terminus of human DNA helicase B fused to tdimer 2 [21] was isolated by digesting the biosensor plasmid with EcoRI, filling in the 5' cleavage site overhang with Klenow enzyme, and then digesting with HindIII.

The probe was complemented with the neo fragment by digesting the pSilencer4.1-CD14shRNA-IRES pSilencer4.1-CD14shRNA-IRES pSilencer4.1-CD14shRNA-IRES

The RFP gene was liberated by digesting the linear plasmid with Cla I and cloned into Nae I/ Cla I sites of pTcINDEX to create pTcINDEX-RFP.

Ac- abf51A was obtained by digesting the recombinant plasmid pGEMT- Ac- abf51A with restriction endonucleases NdeI and NotI (TaKaRa), and was cloned into the NdeI and NotI sites of pET-30a vector (Novagen).

Each SIV containing insert was subsequently removed by digesting the isolated plasmid with Sma I. Vector pFM101d was cut with Eco47 III, dephosphorylated using SAP, gel purified and subsequently the SIV inserts were blunt-end ligated into the Eco47 III site of the gvpC gene contained in the plasmid pFM101d shown in Figure 1.