Sentence examples for by digesting each sample from inspiring English sources

RRLs are generated by digesting each sample with a common restriction enzyme before sequencing and they have been useful for large-scale SNP discovery in several organisms [8] [11].

We generated reduced representation libraries (RRLs) from 17 grapevine DNA samples (10 cultivated V. vinifera varieties, 6 wild Vitis species and the reference genome (inbred Pinot Noir) – see Table S1 for details on samples) by digesting each sample with the restriction enzyme HpaII, which has proved useful in the generation of RRLs by others [26], [27].

Genomic DNA was removed by digesting each sample 20-500 μg of total RNA) with DNase I (Promega).

Genomic DNA was removed by digesting each sample (20 μg of total RNA) with DNaseI (Takara) according to the manufacture's instruction.

Genomic DNA was removed by digesting each sample (10 μg of total RNA) with DNaseI (TIANGEN) according to the manufacture's instruction.

Genomic DNA was removed by digesting each sample (10 μg of total RNA) with Rnase-free DNaseI (Promega) according to the manufacturer's instructions.

We performed a genome complexity reduction step by fully digesting each sample with the restriction enzyme HpaII (recognition sequence = CCGG) to generate reduced representation libraries (RRLs).

The total nitrogen in the soil was determined by Kjeldahl method by digesting soil sample at 390 °C in a digestion tube with 5 ml 98% conc.

The reproducibility (± 5% for most elements) was checked by digesting one sample in triplicate.

Bottom-up workflows overcome these issues by digesting the sample into peptides, which have fewer solubility issues.

Total Kjeldahl nitrogen was estimated by digesting the compost sample in concentrated sulphuric acid and by distillation and trapping the ammonia in 0.1 N sulphuric acid titrated against standard 0.1 N sodium hydroxide solution (AOAC 1995).