Sentence examples for by digesting a purified from inspiring English sources

N-glycosylation was partially characterized by digesting a purified rhGCase sample with PNGase F. MALDI-TOF data processing of the oligosaccharidic chains suggested the presence of 12 glycan forms.

pWY190 was purified from pWY189 by digesting a plasmid preparation with BlpI, which uniquely cleaves pWY189, and then transforming the mixture into E. coli DB3.1 and plating in the presence of spectinomycin.

We tested this by digesting the purified material with several different proteases.

Marker DNA was obtained by digesting purified DNA from the same cells with an appropriate restriction enzyme.

The 6003 bp fragment was recovered by digesting with KpnI, gel purified and ligated into pCaSpeR4 vector (DGRC).

The insert was excised from the plasmid by digesting with NdeI and NotI, purified on a 1% agarose gel, and subcloned into the final pET AT) expression vector, which was also prepared by digesting with NdeI and NotI.

Quantify the plasmid and check the presence of the construct by digesting 1 µg of the purified plasmid as described above.

Double-stranded DNA standards for TNF and EF1α were cloned from C57BL/6 cDNA into the pEF6/TOPO vector (Invitrogen) and then subfragments were generated by restriction digest, purified and quantified using a spectrophotometer (Eppendorf Biophotometer).

In most EcoTILLING protocols heteroduplexed DNA is digested by purified CEL I endonuclease.

Synthetic polypeptides or short fluorogenic peptides are digested by purified 20S proteasomes or cell protein homogenates in 100 μl TEAD buffer (Tris 20 mM, EDTA 1 mM, NaN3 1 mM, DTT 1 mM, pH 7.2) over time at 37°C as previously described (Mishto et al., 2014).

The insert was released by digesting the plasmid with KpnI and EcoRI, purified, ligated into the pRSET expression vector (Invitrogen), and used to transform DH5α cells.