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Correct orientation of the HindIII fragment was verified by digest with SalI and XbaI and DNA sequencing.
The coding sequence for HcRed was removed by digest with AgeI and NotI.
Thereafter, the CCN3/NOV expression cassette was cut out by digest with EcoRI and PmeI, blunted by Klenow, and cloned into vector adenoviral shuttle vector pShuttle-CMV (Stratagene, Agilent Technologies, Waldbronn, Germany).
The RasGAP shRNA sequence was removed from the transgene by digest with Asp718 and XbaI, to leave a pcDNA3.1 backbone (Invitrogen) containing the pol III promoter.
DNA was removed by digest with on-column RNase-free DNase (Qiagen), and RNA was purified and concentrated by RNeasy column (Qiagen).
Similar(54)
Donor islets were isolated by digesting.
The specificity of the amplified product was confirmed by digesting with HhaI.
The polypeptides were cleavaged from GST fusion proteins by digesting with thrombin enzyme.
Wavii creates its newsfeed by digesting information from online news sources and turning them into short summaries.
Species identification was performed by digesting mini-exon PCR products with one or two different restriction enzymes.
The addition of GSH could trigger the decomposition of nanocomposites by digesting MnO2 nanosheets and consequently release Biotin-QDs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com