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Sweat testing is further limited by difficulties in sample recovery (de la Torre and Pichini [2004]).
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The rapid elongation of the inflorescence stem in the model plant Arabidopsis thaliana is accompanied by radical changes in cell wall structure and chemistry, but analysis of the underlying mechanisms and identification of the genes that are involved has been hampered by difficulties in accurately sampling discrete developmental states along the developing stem.
To the major extent it is caused by difficulties in obtaining suitable samples.
The significant potential of tissue-based proteomic biomarker studies can be restricted by difficulties in accessing tissue samples in optimal fresh-frozen form.
Finally, assessment of nodal status is hampered by difficulties in pre-operative sampling, the evidence available suggesting that nodal size is a poor predictor of the presence of metastases.
We lack an accurate characterization of species diversity and phylogeographic patterns in this dominant benthic group--a situation hindered by logistical difficulties in sampling the deep-sea and a persistent shortage of nematode taxonomists.
Structural studies of integral membrane proteins (IMPs) are often hampered by difficulties in producing stable homogenous samples for crystallization.
Unlike studies of marine mammals that use terrestrial habitat, cetacean diet studies are further constrained by difficulty in obtaining samples.
Surveillance programs and research for acute respiratory infections in remote Australian communities are complicated by difficulties in the storage and transport of frozen samples to urban laboratories for testing.
There are additional challenges with investigating airborne respiratory viruses in critically ill patients due to experimental difficulties in sampling aerosolised virus particles, including the potential inactivation of viruses by current sampling methodology [10].
However, our knowledge of the natural history of paediatric HBV infection is severely limited by the difficulty in sampling this group and age-matched controls.
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