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This is exemplified by differential staining using different antibodies to MUC1 and to endothelin.
The purity of isolated neutrophils was determined by differential staining using a commercial May-Grunwald Giemsa stain (Diff-Quick, Baxter Healthcare, UK) and light microscopy and was routinely greater than 97%%.
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We confirmed this differential staining using secondary anti-IgG antibodies alone (not shown).
Fibres can be identified by differential staining and microscopic examination.
Neutrophil percentage was determined by differential staining and cellular differential performed at 100× oil magnification, n = 4 7/group.
Leukocytes also infiltrated the treated mucosa and the number and composition of leukocytes obtained by cervicovaginal lavage (CVL) were determined by differential staining and flow cytometry.
Cryosections (7 µm) obtained with a Leica CM 1850 were stained by differential staining (May Grunwald Giemsa, Bio Optica, Milano, Italy) or by immunocytochemistry to identify myoendothelial cells.
For example, assume a given pair of CTC's can be selected by differential staining.
The goal of this study was to investigate M. tuberculosis in their in vivo environment by using differential staining techniques that target specific components of the M. tuberculosis bacillus, and whether certain bacillary populations could be identified in the different microenvironments tested in vitro and in vivo.
Differential cell counts were stained using Kwick™ Diff and quantified in relation to total cell counts.
Air-dried slides for differential blood count were stained using a Pappenheim stain (Haema Schnellfaerbung, LT-SYS® Labor + Technik Eberhard Lehmann GmbH, Berlin, Germany).
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