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The oxidative stress markers were assayed by differential expression levels of SOD, MDA and Metallothionein (MT) in addition to CTR1, SOD1, COX17, MT and ATOX1 genes expression.
These differences cannot be explained by differential expression levels of PrPsen because by Western blot, PrPsen expression was equivalent.
We found that genes expressed in the node, because it is a small tissue and represents a small fraction of the total RNA, were difficult to detect by differential expression levels.
The heat-tolerant genotype also exhibited a constitutive and specific gene expression pattern, characterised by differential expression levels of a carbohydrate metabolism gene, the fructose-1,6-bisphosphate aldolase (TC176475), which is highly expressed in HS1 at time point 0 h prior to the MHS.
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However, in Caenorhabditis, the rate of synonymous substitution is also strongly affected by the position of a gene in the pathway, and we show that the distribution of selective pressure along the pathway is driven by differential expression level.
Moreover, none of the signals differentiating the two sexes by MALDI profiling corresponded to the theoretical molecular weight of proteins encoded by genes showing differential expression levels based on genomic analyses [4], [6].
It has been experimentally validated that by analyzing the mRNA differential expression levels of putative targets of a transcription factor, changes in the protein-level regulatory activity of that factor can be inferred (Boorsma et al. 2008).
The role of the proteins encoded by the genes showing differential expression levels among the different tested phenotypes revealed that three main genetic processes were involved in color polymorphisms: the biomineralization of the nacreous and calcitic layers and the synthesis of pigments such as melanin in the periostracum.
Among the genes classified as differentially expressed in blastema, 47 regeneration-related genes were selected based on our interests and their differential expression levels were verified by qPCR.
Although the type of immunity activated by Xa39 remains a mystery, the differential expression levels of these hormone regulators suggests that there might be cross-talk between the Xa39-mediated signal transduction cascades and the GA/BR signaling pathway.
Genes with significant differential expression levels were detected by SAM (significance analysis of microarray data) and ANOVA.
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