The mixture of proteins from human lenses was treated by different protocols described in " Materials and methods" (see also in Online Resource for more details for experiments C and D).
However, the possible bias between the relative expression levels and sequences introduced by different protocols of library preparation have rarely been explored.
Constructs were stimulated for 14, 21 and 28 days respectively by different protocols, including cyclic mechanical stimulation, hormonal stimulation or a combination of both.
Results of SM biofilm antimicrobial activity by different protocols were expressed as the kill percentage of biofilm and the term "eradication" was used to denote the kill of 100% of the bacterial population.
The sciatic nerve was dissected free, proximally severed, and electrically stimulated through an electrode by different protocols being controlled by a computer.
To achieve high contrast between inflammatory infiltrates and normal myocardium, it is necessary to suppress myocardial glucose metabolism, which can be obtained by different protocols including prolonged fasting, high-fat low-carbohydrate diet, fatty acid loading, and unfractionated heparin loading [61, 62, 63].
Since the same total RNA extract was used to prepare both libraries, this observation demonstrates the effect of library preparation on the final sequencing data and highlights the difficulty of comparing data generated by different protocols.
In addition, our methods do not currently model biases in RNA-Seq data, and therefore they are not suitable for comparing datasets generated by different protocols.
Tr1 cells can be induced in vitro by different protocols including stimulation with immature dendritic cells (15), IL-10 and/or IFN- α (16), and IL-27 (17– 20), all inducing STAT3 signaling in target T cells [reviewed by Gregori et al. (21)].
