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Both studies demonstrate that elderberry extracts generated by different extraction methods may be useful as alternative or complementary medicines to potentially counteract the spread of certain kinds of bacteria responsible for upper respiratory tract infections.

The volatile oils extracted from PFr, PFo and PCa were diluted to an appropriate concentration so as to obtain a better chromatogram before GC-MS analysis, Figure 3 GC-MS chromatograms of volatile components extracted from different parts of P. frutescens by different extraction methods.

A sensitive fluorescent reagent 2- 11H-benzo[a]carbazol-11-yl) ethyl 4-methylbenzenesulfonate (BCETS) was utilized as pre-column labeling regent to determine fatty acids (FA) from Lycium barbarum seed oils obtained by different extraction methods.

(DOCX 16 KB) 13065_2013_601_MOESM2_ESM.docx Additional file 2: Table S2: HPLC results by different extraction methods.

The extraction method used in this study was one that has been gradually modified based on those studies of extraction, and by examining results obtained by different extraction methods tried over the years.

The main constituents of ylang ylang oil previously obtained by different extraction methods (steam distillation, simultaneous distillation-solvent extraction and supercritical (CO2) extraction) were linalool (16.5 28.0%), germacrene-D (3.1 20.3%), benzyl benzoate (2.9 14.1%), benzyl acetate (6.2 17.0%), caryophyllene (2.9 3.9%) and p-methyl anisole (2.7 6.8%) [45].

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Drosou et al. (2015) compared the extraction yield of air dried Agiorgitico red grape pomace by-products by three different extraction methods using water, water: ethanol (1 1) and ethanol as solvents.

Differences in percentage yields obtained by the different extraction methods did not correlate with activity, since extracts with the highest yield were the least active.

The distribution of S/MARs identified in this study relative to genes, and the differences observed between those isolated by the different extraction methods, suggests that these interactions can be defined and their effects predicted.

The effect on the DNA-recovery by five different extraction methods (n = 50) and the six different blood culture systems (n = 30) was estimated by the copy-number and was assessed by multiple t-tests on log-transformed data (Logn+1) under Bonferroni multiple-comparison-test (LSMEANS analysis associated with PROC GLM, SAS 9.1; SAS Institute, US).

The varied functions of the S/MARs as revealed by the different extraction methods highlights their unique functional contribution.

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